Manual

Antibody List

Antibody Sources and Species Specificities

Citations

1. Stat1 as a component of tumor necrosis factor alpha receptor 1-TRADD signaling complex to inhibit NF-kappaB activation.
2. more to come...

FAQ

• Q: What is AntibodyArray^TM?

  A: AntibodyArray is a recently developed proteomic tool that allows researchers to exam a large number of proteins simultaneously. In AntibodyArrays^TM, a large number of antibodies are arrayed and immobilized on a solid support in a pre-determined order, so that each antibody can be identified later by its position. The immobilized antibodies on the support retain their capabilities of interacting with respective antigens. They recognize and capture the antigens during the incubation with a protein sample. The antigens and their associated protein(s) captured by the arrays can then be studied.

   

• Q: What problems in my research can AntibodyArray^TM help to solve?

  A: Since AntibodyArrays^TM detects proteins, the real players of biological functions, they directly address many difficult questions encountered in biomedical research and drug development. For example, AntibodyArray^TM technology is a powerful but easy-to-use tool for identifying protein-protein interactions, protein posttranslational modifications and protein expressions. The technology can be used to develop an understanding of molecular mechanisms underlying a physiological or pathological process. It can also be used in screening drug candidates.

   

• Q: What advantages does AntibodyArray^TM technology have over other methods, such as yeast two-hybrid screening, in identifying protein-protein interactions?

  A: AntibodyArrays^TM are much faster, less expensive and less subjective to false positive results than yeast two-hybrid screening. More importantly, AntibodyArray^TM technology can detect protein-protein interactions that only occur in the presence of additional cellular factors or only after posttranslational modifications, which yeast two-hybrid screening is not capable of, because those additional factors or modifications do not exist in yeast.

   

• Q: How long does it take to perform an AntibodyArray^TM assay?

  A: Unlike some other methods, such as yeast two-hybrid screening that employs complicated procedures and takes months to perform, the AntibodyArray^TM assay takes less than 6 hrs and use a simple procedure similar to Western blotting.

   

• Q: How sensitive is AntibodyArray^TM technology?

  A: AntibodyArray^TM technology is as sensitive as any other immunological methods. Our customers have routinely detected proteins interacting with low-copy-number cellular proteins.

   

• Q: What makes AntibodyArray^TM technology sensitive and specific?

  A: The high sensitivity of AntibodyArray^TM technology is due to several reasons. First, we carefully selected the antibodies and used the best ones available. Second, the antibodies are at high concentration on the solid support and the high density significantly increases the binding of antigens. Third, because both capturing and detecting antibodies have to bind the same protein complex in order to produce a signal, the chance for false positive result is much lower compared to that in regular immunoassays that only require one antibody binding.

   

• Q: Can I reuse an AntibodyArray^TM?

  A: Yes, you can re-probe a used array with a different antibody. Stripping is possible too. Please call Technical Services at Hypromatrix for more information.

   

• Q: Do I have to purchase expensive equipment to use AntibodyArray^TM?

  A: No. Unlike DNA arrays that require specialized reader to collect and analyze data, AntibodyArray^TM is for routine laboratory use. If you have done a Western blotting, you can perform an AntibodyArray^TM assay.

   

• Q: How is AntibodyArray^TM different from other "protein chip" technology?

  A: One big difference is that AntibodyArray^TM was developed with laboratory researchers in mind. It requires no expensive special equipment.

Related Resources

• PubMed: http://www.ncbi.nlm.nih.gov/PubMed/
• BLAST: http://www.ncbi.nlm.nih.gov/BLAST/
• BINDdatabase: http://bioinfo.mshri.on.ca/
• The Signaling PAthway Database (SPAD): http://bioinfo.mshri.on.ca/ SPAD is an integrated database for genetic information and signal transduction systems.

Technical Contact

Please call 508-856-7900 or email contact@hypromatrix.com