Protein Phosphorylation Profiling
Typical Scenario I
Dr. Frank had been studying the pathology of diabetes
for 5 years. Using a number of different models
including knock-out mice and diabetic rats, he had
generated some very interesting results. However, he
needed a global view of the changes in various signaling
pathways in those models so he could achieve a more
complete picture of different proteins and pathways
involved in the etiology of diabetes. He then came
across AntibodyArraysTM
and was able to utilize this new technique to perform a
fast screening on the phosphorylation patterns of
hundreds of well-studied cellular proteins and found
quite a few interesting molecules.
With protein phosphorylation profiling by
AntibodyArraysTM,
scientists can get a quick read on what proteins have
been phosphorylated (therefore their activities
changed), so they could focus their next stages of
research on proteins that are most likely to be
involved.
Typical Scenario II
A Group Leader in a pharmaceutical company was
deliberating on which lead she should move to the next
stage of development. Her group had identified a number
of chemical entities through high-throughput screening
and found that these molecules bind to the target with
good affinity, but she knew something was missing: She
needed to understand whether those chemicals also have
effects on other cellular proteins. This information
would help her determine the selectivity and toxicity of
drug leads.
She chose to use
AntibodyArraysTM
to help in her assessment of the situation.
By comparing the difference in protein phosphorylations
between untreated cells and cells treated with the drug
leads, she gained insight on the specificity of each
lead and made an informed decision on the next step of
the development.
Technique/Procedure
Detailed Protocol/Manual
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